Isolation and Identification of Bacterial Endophytes from Grain Amaranth (Amaranthus caudatus) for Plant Growth Promotion

Background: Bacterial endophytes lives in internal tissues of root, stem and leaves grain amaranth plants. Isolation screening efficient bacterial for plant growth promotion, biocontrol activities drought tolerance can develop as suitable bioinoculants alleviate stress promotion. Methods: were isolated from (Amaranthus spp) all isolates screened nitrogen fixation, phosphorous solubilisation, hormone production 1-aminocyclopropane-1-carboxylate (ACC) deaminase production. Molecular identification was done by 16S rRNA sequencing to identify the organisms. Result: Seventeen showed high N-free media nine could efficiently solubilize phosphates. Two significantly produced higher concentrations gibberellins, maximum number Indole-3-acetic acid (IAA) eight able produce cytokinins. Fifteen have shown positive ACC activity [1-Aminocyclopropane-1-carboxylate production] five (RAR-5, RAS-3, RAL-7, WAR-1 WAS-11) significant promoting compared others. Further, identified Alcaligenes faecalis subsp. phenolicus, Pseudomonas moorei, Bacillus siamensis, cereus Acromobacter deleyi gene sequencing.